Comparison of an XTT - Based TNF - α Bioassay with a TNF - α ELISA

نویسنده

  • Barbara Manolas
چکیده

1.Bartelt, D.C., S. Fidel, L.H. Farber, D.J. Wolff and R.L. Hammell. 1988. Calmodulin-dependent multifunctional protein kinase in A. nidulans. Proc. Natl. Acad. Sci. USA 85:3279-3283. 2.Bergen, L.G., A. Upshall and N.R. Morris. 1984. S-phase, G2, and nuclear division mutants of Aspergillus nidulans. J. Bacteriol. 159:114-119. 3.Dayton, J.S., M. Sumi, N.N. Namthakumar and A.R. Means. 1997. Expression of a constitutively active Ca2+/calmodulin-dependent kinase in Aspergillus nidulans spores prevents germination and entry into the cell cycle. J. Biol. Chem. 272:3223-3230. 4.Dunbar, B.S. and E.D. Schwoebel. 1990. Preparation of polyclonal antibodies. Methods Enzymol. 182:663-670. 5.Kornstein, L.B., M.L. Gaiso, R.L. Hammell and D.C. Bartelt. 1992. Cloning and sequence determination of a cDNA encoding Aspergillus nidulans calmodulin-dependent multifunctional protein kinase. Gene 113:7582. 6.Kranz, R. and R. Gennis. 1982. A quantitative radioimmunological screening method for specific gene products. Anal. Biochem. 167:247-257. 7.Mink, R.B. and J.A. Johnston. 1996. Quantitative dot-immunoblot assay to measure protein in multiple brain samples. BioTechniques 20:809-811. 8.Parker, C.W. 1990. Immunoassays. Methods Enzymol. 182:700-718. 9.Picard, M.D., G. Litwak, B.C.D. Waite, C.L. Pettey, K.A. Foster and L.J. Thomas. 1996. Sensitive ELISA method for quantitating antibodies to specific protein epitopes. BioTechniques 21:628-630. 10.Schaffner, W. and C. Weissmann. 1973. A rapid, sensitive and specific method for the determination of protein in dilute solution. Anal. Biochem. 56:502-514. 11.Steffen, M.J. and J.L. Ebersole. 1996. Sequential ELISA for cytokine levels in limited volumes of biological fluids. BioTechniques 21:504-509. 12.Timberlake, W.E. 1990. Molecular genetics of Aspergillus development. Annu. Rev. Genet. 24:5-36. 13.Towbin, H.T., T. Staehlin and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Serum TNF-α, IL-10 and IL-2 in Schizophrenic Patients Before and After Treatment with Risperidone and Clozapine

Background: Schizophrenia is a disorder of the executive function of both sensory and central nervous system. Recent studies suggest that immune mechanisms play a role in the pathophysiology of this disease. The variations in cytokine concentrations have been associated with psychopathology and treatment of schizophrenia. Objective: To investigate the changes in serum concentrations of TNF- α, ...

متن کامل

Symptomatic Reactivation of HSV Infection Correlates with Decreased Serum Levels of TNF-α

Background: Herpes simplex viruses (HSV) are human pathogens that establish lytic and latent infections. Reactivation from latency occurs intermittently, which represents a life-long source for recurrent infection. The role of immune factors in the control of recurrent symptomatic HSV lesions is complex and the exact role of cytokines remains unclear. Objective: To assess the levels of tumor ne...

متن کامل

Effect of sitagliptin on serum levels of TNF-α, IL-1β and IL-10 in patients with type 2 diabetes mellitus

Introduction: Type 2 diabetes mellitus (T2DM) is an inflammatory disease with alterations in immune system. Remarkably, cytokine imbalance plays an important role in pathogenesis of T2DM. The purpose of this study was to determine the serum levels of TNF-α, IL-1β and IL-10 and the effects of sitagliptin on the level of these cytokines in patients with T2DM. Materials and Methods: Blood samples ...

متن کامل

Comparison of cytokines IL10, IFN-Y, TNF-α, TGF-β and antioxidant enzymes glutathione reductase and glutathione peroxidase in saliva and serum of Iranian patients with alopecia totalis

The aim of this study was to investigate the possibility of using saliva instead of serum (as a simpler method without the need for blood sampling) to determine the level of some cytokines and enzymes. In this study, the levels of cytokines IL10, IFN-Y, TNF-α, TGF-β and the antioxidant enzymes glutathione reductase and glutathione peroxidase were measured in the saliva and serum of three patien...

متن کامل

Characterization of Novel Fragment Antibodies Against TNF-alpha Isolated Using Phage Display Technique

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine which plays crucial roles in pathogenesis of inflammatory diseases. The current study aimed to investigate the binding abilities of I44 and I49 domain antibodies to TNF-α. The dAbs were expressed in bacterial expression system and purified by affinity chromatography using Ni-sepharose column. The expression and purity of the protei...

متن کامل

Characterization of Novel Fragment Antibodies Against TNF-alpha Isolated Using Phage Display Technique

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine which plays crucial roles in pathogenesis of inflammatory diseases. The current study aimed to investigate the binding abilities of I44 and I49 domain antibodies to TNF-α. The dAbs were expressed in bacterial expression system and purified by affinity chromatography using Ni-sepharose column. The expression and purity of the protei...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 1999